Difference between revisions of "Part:BBa K649105"
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Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR). | Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR). | ||
+ | [[Image:OD lsrR repression.png|thumb|center|400px|After four hours from OD590 reaching 0.15, we measured OD.<br>This work is done by Hiroki Yoshise.]] | ||
[[Image:LsrR repression.png|thumb|center|300px|Median fluorescence intensity(MFI) is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]] | [[Image:LsrR repression.png|thumb|center|300px|Median fluorescence intensity(MFI) is decreased by LsrR repression.<br>This work is done by Hiroki Yoshise.]] |
Revision as of 09:31, 2 October 2011
PlsrA-gfp-PlsrR-lsrR
Median fluorescence intensity(MFI) of BBa_K649104(PlsrA-RBS-gfp) is 3-fold higher than that of BBa_K649105(PlsrA-gfp-PlsrR-lsrR).
We confirmed that LsrR represses lsrA promoter.
For this purpose, we constructed BBa_K649105(PlsrA-gfp-PlsrR-lsrR) and compared fluorescence intensity levels of BBa_K649104(PlsrA-RBS-gfp) and BBa_K649105.
For more information, see our work in Tokyo_Tech 2011 wiki
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 2579
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2253
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 770