Difference between revisions of "Part:BBa K649301"
Line 8: Line 8:
  
 
You should NOT ligate this part into high-copy vector.<br />
 
You should NOT ligate this part into high-copy vector.<br />
−
In our test, this part ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow after 24 hours incubation.<br />
+
In our test, when this part was ligated into pSB1C3, <span style="font-style:italic;">E. coli</span> DH5&alpha; didn't glow even after 24 hours incubation.<br />
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:00, 3 October 2011

Ptrc-RBS-rocF

Arginase is coded with Ptrc and RBS in this part.

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.
In our test, when this part was ligated into pSB1C3, E. coli DH5α didn't glow even after 24 hours incubation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]