Difference between revisions of "Part:BBa I723020:Experience"

Revision as of 15:58, 1 October 2011

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Applications of BBa_I723020

User Reviews

UNIQ5bcc4faa9f0a8783-partinfo-00000000-QINU

BBa_I723020 1 Not understood Sebastian Murmann ETH Zürich 2011

We had some troubles to get the part out of the iGEM spring distribution 2011. Although trying for several times, we did not succeed in transforming the plasmid provided in the distribution. Thus we used the plasmid pCK04YxylR as source for the promoter. We further modified the part by adding a double stop codon to it, resulting in BBa_K625002. The second modification was to remove the remaining sequence of xylU, which resulted in the short promoter variant BBa_K625003. The characterization and comparison of the modified promoters can be found in the characterization section of BBa_K625002 respectively BBa_K625003.

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-We had some troubles to get the part out of the iGEM spring distribution 2011. Although trying for several times, we did not succeed in transforming the plasmid provided in the distribution. Thus we used the plasmid ''pCK04YxylR'' as source for the promoter. We further modified the part by adding a double stop codon to it, resulting in [https://parts.igem.org/Part:BBa_K625002 BBa_K625002]. The second modification was to remove the remaining sequence of ''xylU'', which resulted in the short promoter variant [https://parts.igem.org/Part:BBa_K625002 BBa_K625003]. The characterization and comparison of the new promoters can be found in the characterization section of [https://parts.igem.org/Part:BBa_K625002 BBa_K625002] respectively [https://parts.igem.org/Part:BBa_K625003 BBa_K625003].
+We had some troubles to get the part out of the iGEM spring distribution 2011. Although trying for several times, we did not succeed in transforming the plasmid provided in the distribution. Thus we used the plasmid ''pCK04YxylR'' as source for the promoter. We further modified the part by adding a double stop codon to it, resulting in [https://parts.igem.org/Part:BBa_K625002 BBa_K625002]. The second modification was to remove the remaining sequence of ''xylU'', which resulted in the short promoter variant [https://parts.igem.org/Part:BBa_K625002 BBa_K625003]. The characterization and comparison of the modified promoters can be found in the characterization section of [https://parts.igem.org/Part:BBa_K625002 BBa_K625002] respectively [https://parts.igem.org/Part:BBa_K625003 BBa_K625003].
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