Difference between revisions of "Part:BBa K676004:Design"

Latest revision as of 21:07, 1 October 2011

Supercoilometer

Assembly Compatibility:

Device constructed by performing 3A assembly between BBa_K676003 (TyrT promoter) and BBa_E0422 (RBA+eCFP+Ter) and pSB1C3 plasmid backbone.

TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M).

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 TyrT promoter from E coli genome and CFP expression casette from iGEM kit plate (Spring Distribution 2011 Kit Plate 1 - 8M).
-===References===
-. Straney, R., R. Krah, and R. Menzel, Mutations in the -10 Tataat Sequence of the Gyra Promoter Affect Both Promoter Strength and Sensitivity to DNA Supercoiling. Journal of Bacteriology, 1994. 176(19): p. 5999-6006.
-. Pemberton, I.K., et al., The G+C-rich discriminator region of the tyrT promoter antagonises the formation of stable preinitiation complexes. Journal of Molecular Biology, 2000. 299(4): p. 859-864.
-. Lamond, A.I., Supercoiling response of a bacterial tRNA gene. Embo Journal, 1985. 4(2): p. 501-7.
-. Bowater, R.P., D.R. Chen, and D.M.J. Lilley, Modulation of Tyrt Promoter Activity by Template Supercoiling in-Vivo. Embo Journal, 1994. 13(23): p. 5647-5655.
-. El Hanafi, D. and L. Bossi, Activation and silencing of leu-500 promoter by transcription-induced DNA supercoiling in the Salmonella chromosome. Molecular Microbiology, 2000. 37(3): p. 583-594.
-. Bossi, L., et al., The supercoiling sensitivity of a bacterial tRNA promoter parallels its responsiveness to stringent control. Embo Journal, 1998. 17(8): p. 2359-2367.