Difference between revisions of "Part:BBa K549001:Experience"
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Our first experiment with this BioBrick showed positive results. Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer.  
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'''Our first experiment with this BioBrick showed positive results.'''
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Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer.  
 
For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.  
 
For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.  
  
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[[Image:RcnA_test.png|800px|center|alt phase contrast microscopy showing with BBa_K549001 transformed E.coli cells]]
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[[Image:RcnA_test.png|800px|center]]
  
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''Figure 1'': with BBa_K549001 transformed stationary phase ''E.coli'' cells, cultivated in LB-media (containing Ni(II)) a) left hand side: phase contrast microscopy b)right-hand side: fluorescence microscopy GFP-excitation
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Transformed and induced ''E. coli'' cells, cultivated in LB-media (containing Ni(II)). left-hand side: phase contrast right-hand side: GFP-excitation;
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===Applications of BBa_K549001===
 
===Applications of BBa_K549001===

Revision as of 18:02, 30 September 2011


Our first experiment with this BioBrick showed positive results. Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer. For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.

RcnA test.png

Figure 1: with BBa_K549001 transformed stationary phase E.coli cells, cultivated in LB-media (containing Ni(II)) a) left hand side: phase contrast microscopy b)right-hand side: fluorescence microscopy GFP-excitation

Applications of BBa_K549001

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