Difference between revisions of "Part:BBa K549001:Experience"
| Line 4: | Line 4: | ||
For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output. | For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output. | ||
| − | [[Image: | + | [[Image:RcnA_test.png|800px|center|alt phase contrast microscopy showing with BBa_K549001 transformed E.coli cells]] |
| + | |||
Transformed and induced ''E. coli'' cells, cultivated in LB-media (containing Ni(II)). left-hand side: phase contrast right-hand side: GFP-excitation; | Transformed and induced ''E. coli'' cells, cultivated in LB-media (containing Ni(II)). left-hand side: phase contrast right-hand side: GFP-excitation; | ||
Revision as of 17:52, 30 September 2011
Our first experiment with this BioBrick showed positive results. Hypothetically the linear response for this system is fulfilled for a concentration range from 0.5 to 60 µM. But only to see, whether the BioBrick works or not, we started a pretest to see a yes-or-no-answer.
For this we induced the system with a nickel-concentration lying high above the said concentration range and measured the output when the colony was in its stationary phase. As you can clearly see here in figure 1b), nearly all of the cells gave an apparent output.
Transformed and induced E. coli cells, cultivated in LB-media (containing Ni(II)). left-hand side: phase contrast right-hand side: GFP-excitation;
Applications of BBa_K549001
User Reviews
UNIQ58fbc41745b1a1c4-partinfo-00000000-QINU UNIQ58fbc41745b1a1c4-partinfo-00000001-QINU