Difference between revisions of "Part:BBa K560000"
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This part was designed to build the complete blue light sensing and reporting system in a single plasmid. Promoter BBa_J23117 is a constitutive promoter and not a strong one. It will keep a certain concentration of LovTAP in bacteria. When exposure to blue light, the absorption of a photon will leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.
 
This part was designed to build the complete blue light sensing and reporting system in a single plasmid. Promoter BBa_J23117 is a constitutive promoter and not a strong one. It will keep a certain concentration of LovTAP in bacteria. When exposure to blue light, the absorption of a photon will leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.
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[[Image:Whu-Part1.png]]
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[[Image:Whu-Part1-1.png]]
  
  

Revision as of 15:06, 30 September 2011

Blue Light Sensor+PtrpL+mRFP1

Blue Light Sensing and Reporting System

This part was designed to build the complete blue light sensing and reporting system in a single plasmid. Promoter BBa_J23117 is a constitutive promoter and not a strong one. It will keep a certain concentration of LovTAP in bacteria. When exposure to blue light, the absorption of a photon will leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter. Whu-Part1-1.png



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 595


Functional Parameters

n/a Blue Light Sensor+PtrpL+mRFP1