Difference between revisions of "Part:BBa K646004:Design"

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===Design Notes===
 
===Design Notes===
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Using parts BBa_K646000 and BBa_I719004, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available.
+
Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available.
  
 
Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1).
 
Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1).

Latest revision as of 13:25, 30 September 2011

T7 promoter with Hemeoxygenase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Using parts BBa_K646000 and BBa_I712074, a T7 promoted Hemeoxygenase was created by digesting the T7 biobrick with SpeI and PstI and ligating it to the HO insert digested with XbaI and PstI. This procedure can be used if an EcoRI and PstI cut plasmid backbone is not available.

Successful expression in E.coli should result in green colonies due to the presence of biliverdin (synthesized by heme oxygenase 1).

Source

BBa_K646000 and BBa_I712074

References