Difference between revisions of "Part:BBa K625002"
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<partinfo>BBa_K625002 short</partinfo>
 
<partinfo>BBa_K625002 short</partinfo>
  
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This is an adapted version of the Pu promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_I723020 BBa_I723020]. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this biobrick is cloned without scar), a double stop codon was inserted at the end of the promoter.
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This is an adapted version of the Pu promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_I723020 BBa_I723020]. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this BioBrick is cloned without scar), a double stop codon was inserted at the end of the promoter.
  
 
==='''Characterization'''===
 
==='''Characterization'''===
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===Experimental setup===
 
===Experimental setup===
  
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A
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''E. coli'' strain JM101 was transformed with two plasmids containing the transcriptional regulator XylR, the degradation cassette ''xylWABN'' and a GFP reporter coupled to [https://parts.igem.org/Part:BBa_K625002 BBa_K625002] respectively [https://parts.igem.org/Part:BBa_K625003 BBa_K625003].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 10:03, 30 September 2011

Pu promoter long version with stop codon

This is an adapted version of the Pu promoter BBa_I723020. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this BioBrick is cloned without scar), a double stop codon was inserted at the end of the promoter.

Characterization

Experimental setup

E. coli strain JM101 was transformed with two plasmids containing the transcriptional regulator XylR, the degradation cassette xylWABN and a GFP reporter coupled to BBa_K625002 respectively BBa_K625003.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]