Difference between revisions of "Part:BBa K567018:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<partinfo>BBa_K567018 AddReview 2</partinfo>
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<I>kosyumote</I>
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The Pitt iGEM 2015 team characterized this part in NiCo21(DE3) cells (a BL21(DE3) derivative). This part exhibits significant leaky expression due to the lac operator present in high copy number. For applications where leaky expression is deleterious, this part does not work. However, it produces easily quantifiable GFP which can be useful in many applications. Finally, we did not expect RFP fluorescence in NiCo21(DE3) or DH5a cells, since neither of them have amber suppressors, and none was observed.
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Revision as of 18:51, 18 September 2015

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K567018

User Reviews

UNIQe6ba3ce81bdb4461-partinfo-00000000-QINU

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kosyumote

The Pitt iGEM 2015 team characterized this part in NiCo21(DE3) cells (a BL21(DE3) derivative). This part exhibits significant leaky expression due to the lac operator present in high copy number. For applications where leaky expression is deleterious, this part does not work. However, it produces easily quantifiable GFP which can be useful in many applications. Finally, we did not expect RFP fluorescence in NiCo21(DE3) or DH5a cells, since neither of them have amber suppressors, and none was observed.

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UNIQe6ba3ce81bdb4461-partinfo-00000002-QINU