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The graph clearly shows that expression has been significantly induced by white light for expression with the bluf domain. When averaged out, the total induction shows an increase in YFP fluorescence by a 2.4 fold level, however, the promoter does exhibit some leaky expression. | The graph clearly shows that expression has been significantly induced by white light for expression with the bluf domain. When averaged out, the total induction shows an increase in YFP fluorescence by a 2.4 fold level, however, the promoter does exhibit some leaky expression. | ||
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| + | The construct used to test fluorescence is available here (https://parts.igem.org/wiki/index.php?title=Part:BBa_K660601) | ||
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Revision as of 00:50, 30 September 2011
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Applications of BBa_K238013
User Reviews
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BBa_K238013 Glasgow iGEM 2011 |
Glasgow iGEM 2011 We coupled the bluf promoter to YFP and measured intensity after induction with white light. We found that expression showed a 2.4* increase in fold induction when compared with darkness as a control. The promoter demonstrated leaky expression with no light induction.
The image above demonstrates expression of YFP ligated to an RBS and the bluf promoter. The images are taken from two samples, one which was grown in darkness and the other grown with light present. The samples were shaken overnight at room temperature and the intensity of expression was measured. (See graph below) Scale = 10 microns. 1st Fluorescence filter : 515-565nm (left column) 2nd Fluorescence filter : 470/20 nm (right column)
The graph clearly shows that expression has been significantly induced by white light for expression with the bluf domain. When averaged out, the total induction shows an increase in YFP fluorescence by a 2.4 fold level, however, the promoter does exhibit some leaky expression. The construct used to test fluorescence is available here (https://parts.igem.org/wiki/index.php?title=Part:BBa_K660601) |
UNIQ2634b085f3716627-partinfo-00000002-QINU