Difference between revisions of "Part:BBa J176014"
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3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA (Chung NAR 2006) | 3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA (Chung NAR 2006) | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here --> |
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 22:41, 29 September 2011
Intron
3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA (Chung NAR 2006)
Usage and Biology
REFERENCES:
- FChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1176
Illegal XhoI site found at 1253 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 139