Difference between revisions of "Part:BBa J176014"
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===Usage and Biology===
 
===Usage and Biology===
  
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REFERENCES:<!-- Last FN, Last2 FN2. (year) Title. Journal abbrev. Vol:p1-p100. -->
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REFERENCES:
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# FChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
 
# FChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444
  

Revision as of 22:40, 29 September 2011

Intron

3 exons & 2 introns; intron 2 has SIBR cassette for Bbsl insertion of miRNA (Chung NAR 2006)

  1. FChung KH, Hart CC, Al-Bassam S, Avery A, Taylor J, Patel PD, Vojtek AB, Turner DL. (2006) Polycistronic RNA polymerase II expression vectors for RNA interference based on BIC/miR-155. NAR. 34:e53. PMID: 16614444

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1176
    Illegal XhoI site found at 1253
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 139