Difference between revisions of "Part:BBa K640003"

Latest revision as of 20:20, 29 September 2011

Ori1600- Pseudomonas origin of replication

ori1600 was initially thought to be a replication stabilizing element for Pseudomonas spp. Later, it was confirmed that it is a replication origin for plasmids replicating in Pseudomonas spp. Together with our part BBa_K640002 (oriT for interspecies transfer), a plasmid containing these parts can be transferred to Pseudomonas spp. and is able to replicate.

Sequence and Features

Functionality Test

In our first assay, we wanted to see that our BBa_K640004 (oriT-ori1600) construct was working. We incubated the co-cutures for 12 hours at 37°c prior to plating. The plates were then left to grow overnight.

Results

The large plates on the right show controls of Pseudomonas and E. coli on MacConkey plates indicating the normal expected colors for these organisms. The small plates on the bottom represent our construct, conjugated with two different strains of pseudomonas and plated on MacConkey agar with Kanamycin (50ug/mL). The yellow color indicates that the colonies are Pseudomonas. The small plates on the top show our two strains of Pseudomonas, as well as E. coli without kanamycin resistance plated on MacConkey agar plates with kanamycin (50 ug/mL). All of these plates are pink in color, and show no colonies. This was expected, as it shows that without a resistance marker, these organisms are not able to grow on the MacConkey kanamycin plates. This demonstrates that our part is working the way we expect it to!