Difference between revisions of "Part:BBa K672000"

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[[Image:BBCOMBINATION.jpg]]
 
[[Image:BBCOMBINATION.jpg]]
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==Characterization & Funtioning Experience==
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===Experiments with BBa_K672000===
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We designed the following experiment in order to: test the BioBrick operation in different substrates and temperatures, characterize the expression of the promoters, determine the strongest promoter for the device operation, define the most efficient temperature and substrate for the operation of this device, provide a way to survive at low temperatures and be able to measure nitrate.
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We chose 4 temperatures:
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• 5-10°C
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• 10-20°C
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• 20-30°C
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• 37°C
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These temperatures were selected so we could evaluate the response to cold shock in various ranges for our BioBrick. Since the lab equipments we had available couldn’t reach temperatures between 10-20°C this temperature was not used during the experience. In the end, the lab equipment allowed us to make the experiments in: 37°C, 23°C and 8°C.
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We also chose 3 different Growth Media:
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• Saline Solution
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• Liquid LB
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• Minimal Media
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These growth medias where selected to evaluate the response of our BioBricks in different metabolic pathways.
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'''We did Spectrophotometry measurments, obtaining the following results:'''<br><br>
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[[Image:renbo.jpg|center]]<br>
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LB stands for Luria Broth; MM, for Minimal Media and SS, for Saline Solution.
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'''Which leads us to the following conclusions:'''<br>
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# The best growth is obtained in Luria Broth. <br>
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# Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.
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===Experiments with Other BioBricks===
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[[Image:bristol.jpg|center]]
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[[Image:gatech.jpg|center]]
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===User Reviews===
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<!-- DON'T DELETE --><partinfo>BBa_K672000 StartReviews</partinfo>
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<!-- Template for a user review
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{|width='80%' style='border:1px solid gray'
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|-
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|width='10%'|
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<partinfo>BBa_K672000 AddReview number</partinfo>
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<I>Username</I>
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|width='60%' valign='top'|
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Enter the review inofrmation here.
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|};
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<!-- End of the user review template -->
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<!-- DON'T DELETE --><partinfo>BBa_K672000 EndReviews</partinfo>

Revision as of 07:23, 29 September 2011

THERMOGENIC RESPONSE NUTRIENT BIOSENSOR

The Bristol BBa_K381001 encodes an expression of GFP in response of Nitrate or Nitrites that expresses fluorescent signals upon nutrient detection, its principal application is that allows farmers to quantify soil nutrient content. The BBa_K410000 is a fusion of the HydB cold shock promoter, the OmpA a signal peptide, and AOX 1 . AOXa is a alternative oxidase found in Sacred Lotus. This is going to produce a increase of heat above ambient temperature as follow:

· From 20°C to 30°C the HydB promoter will express. · Approximately at 37°C the promoter will stop his expression.

By combining BBa_K381001 and BBa_K410000 we expected our part , the BBa_K672000 , to be able to sense nitrate and nitrites even after experiencing a cold shock at 20ºC, making our BB employable in other places where the temperatures can be as low as 15ºC.

SubParts:

BBCOMBINATION.jpg

Characterization & Funtioning Experience

Experiments with BBa_K672000

We designed the following experiment in order to: test the BioBrick operation in different substrates and temperatures, characterize the expression of the promoters, determine the strongest promoter for the device operation, define the most efficient temperature and substrate for the operation of this device, provide a way to survive at low temperatures and be able to measure nitrate.


We chose 4 temperatures:

• 5-10°C

• 10-20°C

• 20-30°C

• 37°C


These temperatures were selected so we could evaluate the response to cold shock in various ranges for our BioBrick. Since the lab equipments we had available couldn’t reach temperatures between 10-20°C this temperature was not used during the experience. In the end, the lab equipment allowed us to make the experiments in: 37°C, 23°C and 8°C.

We also chose 3 different Growth Media:

• Saline Solution

• Liquid LB

• Minimal Media


These growth medias where selected to evaluate the response of our BioBricks in different metabolic pathways.


We did Spectrophotometry measurments, obtaining the following results:

Renbo.jpg

LB stands for Luria Broth; MM, for Minimal Media and SS, for Saline Solution.

Which leads us to the following conclusions:

  1. The best growth is obtained in Luria Broth.
  2. Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.

Experiments with Other BioBricks

Bristol.jpg
Gatech.jpg

User Reviews

UNIQ90e007f9c0bf13b1-partinfo-00000000-QINU UNIQ90e007f9c0bf13b1-partinfo-00000001-QINU