Difference between revisions of "Part:BBa K672000"
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== '''THERMOGENIC RESPONSE NUTRIENT BIOSENSOR''' == | == '''THERMOGENIC RESPONSE NUTRIENT BIOSENSOR''' == | ||
| − | The Bristol | + | The Bristol BBa_K381001 encodes an expression of GFP in response of Nitrate or Nitrites that expresses fluorescent signals upon nutrient detection, its principal application is that allows farmers to quantify soil nutrient content. The BBa_K410000 is a fusion of the HydB cold shock promoter, the OmpA a signal peptide, and AOX 1 . AOXa is a alternative oxidase found in Sacred Lotus. This is going to produce a increase of heat above ambient temperature as follow: |
· From 20°C to 30°C the HydB promoter will express. | · From 20°C to 30°C the HydB promoter will express. | ||
· Approximately at 37°C the promoter will stop his expression. | · Approximately at 37°C the promoter will stop his expression. | ||
| − | By combining | + | By combining BBa_K381001 and BBa_K410000 we expected our part , the BBa_K672000 , to be able to sense nitrate and nitrites even after experiencing a cold shock at 20ºC, making our BB employable in other places where the temperatures can be as low as 15ºC. |
== '''SubParts:''' == | == '''SubParts:''' == | ||
[[Image:BBCOMBINATION.jpg]] | [[Image:BBCOMBINATION.jpg]] | ||
Revision as of 06:36, 29 September 2011
THERMOGENIC RESPONSE NUTRIENT BIOSENSOR
The Bristol BBa_K381001 encodes an expression of GFP in response of Nitrate or Nitrites that expresses fluorescent signals upon nutrient detection, its principal application is that allows farmers to quantify soil nutrient content. The BBa_K410000 is a fusion of the HydB cold shock promoter, the OmpA a signal peptide, and AOX 1 . AOXa is a alternative oxidase found in Sacred Lotus. This is going to produce a increase of heat above ambient temperature as follow:
· From 20°C to 30°C the HydB promoter will express. · Approximately at 37°C the promoter will stop his expression.
By combining BBa_K381001 and BBa_K410000 we expected our part , the BBa_K672000 , to be able to sense nitrate and nitrites even after experiencing a cold shock at 20ºC, making our BB employable in other places where the temperatures can be as low as 15ºC.
SubParts:
