Difference between revisions of "Part:BBa K672000:Experience"

Revision as of 05:32, 29 September 2011

Experiments with BBa_K672000

We did Spectrophotometry measurments, obtaining the following results:

Which leads us to the following conclusions:

The best growth is obtained in Luria Broth.
Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.

Experiments with Other BioBricks

User Reviews

UNIQ4862f5f3d68909b2-partinfo-00000000-QINU UNIQ4862f5f3d68909b2-partinfo-00000001-QINU

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 ===Experiments with Other BioBricks===
+[[Image:bristol.jpg]]
-BBa_K381001 Bristol
+[[Image:gatech.jpg]]
-Sample ID	        Temperature (°C)	Substrate 	Absorbance @420 nm
-BBa_K381001 Bristol    	     23	                   LB	              0.599
-BBa_K381001 Bristol	     37	                   LB	              0.961
-BBa_K381001 Bristol	     8	                   LB	              0.015
-BBa_K381001 Bristol	     23                    MM	              0.341
-BBa_K381001 Bristol	     37	                   MM	              0.351
-BBa_K381001 Bristol	     8	                   MM	              0.021
-BBa_K381001 Bristol	     23	                   SS	              0.010
-BBa_K381001 Bristol	     37	                   SS	              0.006
-BBa_K381001 Bristol	     8	                   SS	              0.005
-From these data we could conclude that:
-•	The BB grows best in LB.
-•	It grows less in Minimal Media.
-•	It shows almost no growth in Saline Solution.
-•	Il grows best @37°C
-•	It grows good @23°C
-•	It shows almost no growth @8°C
-BBa_K410000 GaTech
-Sample ID	     Temperature (°C)	Substrate 	Absorbance @420 nm
-BBa_K410000 GaTech	  23	            LB                0.258
-BBa_K410000 GaTech	  37	            LB         	      0.624
-BBa_K410000 GaTech	  8	            LB	              0.006
-BBa_K410000 GaTech	  23	            MM	              0.275
-BBa_K410000 GaTech	  37	            MM	              0.028
-BBa_K410000 GaTech	  8	            MM	              0.004
-BBa_K410000 GaTech	  23	            SS	              0.015
-BBa_K410000 GaTech	  37	            SS	             -0.001
-BBa_K410000 GaTech	  8	            SS	              0.014
-From these data we could conclude that:
-•	The BB grows best in LB.
-•	It grows well in Minimal Media.
-•	It shows almost no growth in Saline Solution.
-•	Il grows best @37°C
-•	It grows well @23°C (best in Minimal Media than LB)
-•	It shows almost no growth @8°C
 ===User Reviews===