Difference between revisions of "Part:BBa K672000:Experience"

Revision as of 05:24, 29 September 2011

Experiments with BBa_K672000

We did Spectrophotometry measurments, obtaining the following results:

Which leads us to the following conclusions:

The best growth is obtained in Luria Broth.
Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.

Experiments with Other BioBricks

BBa_K381001 Bristol

Sample ID Temperature (°C) Substrate Absorbance @420 nm BBa_K381001 Bristol 23 LB 0.599 BBa_K381001 Bristol 37 LB 0.961 BBa_K381001 Bristol 8 LB 0.015 BBa_K381001 Bristol 23 MM 0.341 BBa_K381001 Bristol 37 MM 0.351 BBa_K381001 Bristol 8 MM 0.021 BBa_K381001 Bristol 23 SS 0.010 BBa_K381001 Bristol 37 SS 0.006 BBa_K381001 Bristol 8 SS 0.005

From these data we could conclude that: • The BB grows best in LB. • It grows less in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows good @23°C • It shows almost no growth @8°C

BBa_K410000 GaTech

Sample ID Temperature (°C) Substrate Absorbance @420 nm BBa_K410000 GaTech 23 LB 0.258 BBa_K410000 GaTech 37 LB 0.624 BBa_K410000 GaTech 8 LB 0.006 BBa_K410000 GaTech 23 MM 0.275 BBa_K410000 GaTech 37 MM 0.028 BBa_K410000 GaTech 8 MM 0.004 BBa_K410000 GaTech 23 SS 0.015 BBa_K410000 GaTech 37 SS -0.001 BBa_K410000 GaTech 8 SS 0.014

From these data we could conclude that: • The BB grows best in LB. • It grows well in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows well @23°C (best in Minimal Media than LB) • It shows almost no growth @8°C

User Reviews

UNIQ8e7ca84887ed1c00-partinfo-00000000-QINU UNIQ8e7ca84887ed1c00-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 # The best growth is obtained in Luria Broth. <br>
 # Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.
 ===Experiments with Other BioBricks===