Difference between revisions of "Part:BBa K542003"

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Intermediate part to assemble the "Co-localization Construct".  
 
Intermediate part to assemble the "Co-localization Construct".  
 
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This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct. The data sheet for BBa_K542008 is shown below.  
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This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.  
 
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[[image:uoflECdatasheet.png|150px]]  
 
[[image:uoflECdatasheet.png|150px]]  

Revision as of 00:44, 29 September 2011

pBAD Regulated TetR

Made by assembling BBa_I13453 with BBa_P0440.

Production of TetR is regulated by the pBAD promoter (BBa_I13453). TetR is an inhibitor of the constitutively "on" promoter pTet--BBa_R0040). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")

Intermediate part to assemble the "Co-localization Construct".

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

UoflECdatasheet.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]