Difference between revisions of "Part:BBa K569013:Design"
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===Source=== | ===Source=== | ||
− | + | ToxR-- J07009 (MIT) | |
+ | |||
+ | FGFR-- Received from Openbiosystems | ||
+ | |||
+ | PyeaR-- BBa_K216005 (Edinburgh) | ||
===References=== | ===References=== |
Latest revision as of 00:12, 29 September 2011
PyeaR+ToxR+FGFR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1331
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 909
Illegal NgoMIV site found at 1500 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
FGFR
1. Added restriction sites XbaI and SpeI via PCR mechanism 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
Source
ToxR-- J07009 (MIT)
FGFR-- Received from Openbiosystems
PyeaR-- BBa_K216005 (Edinburgh)