Difference between revisions of "Part:BBa K569014:Design"
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FGFR-- Received from Openbiosystems | FGFR-- Received from Openbiosystems | ||
− | Added restriction sites XbaI and SpeI via PCR mechanism | + | 1. Added restriction sites XbaI and SpeI via PCR mechanism |
− | Did a point mutation experiment because there was an EcoRI restriction site within the gene | + | 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene |
Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119 | Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119 |
Revision as of 23:58, 28 September 2011
SCP+ToxR+FGFR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 844
Illegal NgoMIV site found at 1435 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
ToxR-- Received from iGEM, part J07009
FGFR-- Received from Openbiosystems
1. Added restriction sites XbaI and SpeI via PCR mechanism 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
Strong Constitutive Promoter-- Received from iGEM, part BBa_J23119
Source
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