Difference between revisions of "Part:BBa K511814"

 
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<partinfo>BBa_K511814 short</partinfo>
 
<partinfo>BBa_K511814 short</partinfo>
  
 
This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces Delta-mCherry at a low, basal (OFF) level.
 
This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces Delta-mCherry at a low, basal (OFF) level.
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Here is a graph showing doxycycline dependent activation of TRE_Delta-mCherry in the presence of constitutively expressed rtTA3.
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https://static.igem.org/mediawiki/parts/8/8f/Tre_delta.jpg
  
  

Revision as of 15:42, 5 November 2011

Inducible Red Fluorescent Ligand Generator (TRE-Tight-Delta-mCherry) MammoBlock Device

This MammoBlock composite device produces the red fluorescent juxtacrine signaling ligand Delta-mCherry when induced with tTA/rtTA transactivator variants in the presence of tetracycline analogues, and otherwise produces Delta-mCherry at a low, basal (OFF) level.

Here is a graph showing doxycycline dependent activation of TRE_Delta-mCherry in the presence of constitutively expressed rtTA3.

Tre_delta.jpg


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
    Illegal NotI site found at 846
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 6
    Illegal EcoRI site found at 322
    Illegal XbaI site found at 30
    Illegal PstI site found at 437
    Illegal PstI site found at 514
    Illegal PstI site found at 1357
    Illegal PstI site found at 1780
    Illegal PstI site found at 2457
    Illegal PstI site found at 2843
    Illegal AgeI site found at 873
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 385
    Illegal BsaI site found at 825
    Illegal BsaI.rc site found at 2090
    Illegal BsaI.rc site found at 2262
    Illegal SapI site found at 1578
    Illegal SapI.rc site found at 2350