Difference between revisions of "Part:BBa K652000"

Revision as of 21:49, 28 September 2011

T7-RBS-eGFP-TEV-RiAFP-His-Term

This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Rhagium inquistor antifreeze protein (RiAFP) with a His tag at the C-terminus, followed by a terminator.

More information about this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale

Cloning results were confirmed by colony PCR, restriction digest, and full sequencing.

Expression levels of up to 150mg/L of this protein are detected by UV-vis.

Figure 1: Expression of RiAFP-GFP for purification. File:Jen RiGFP Purification (08-04-2011).jpg

Figure 2-3: Cleavage at TEV site for isolation of RiAFP

Figure 4: RiAFP-GFP increases survivability of E. coli

Figure 5: RiAFP-GFP inhibits ice recrystallization

Figure 6: RiAFP-GFP enhances

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 894
Illegal AgeI site found at 990
Illegal AgeI site found at 1104
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 74
Illegal SapI.rc site found at 87

@@ Line 6: / Line 6: @@
 More information about this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale
-Figure 1: Gel confirming
+Cloning results were confirmed by colony PCR, restriction digest, and full sequencing.
+Expression levels of up to 150mg/L of this protein are detected by UV-vis.
+Figure 1: Expression of RiAFP-GFP for purification.
+[[Image:Jen RiGFP Purification (08-04-2011).jpg]]
+Figure 2-3: Cleavage at TEV site for isolation of RiAFP
+Figure 4: RiAFP-GFP increases survivability of E. coli
+Figure 5: RiAFP-GFP inhibits ice recrystallization
+Figure 6: RiAFP-GFP enhances