Difference between revisions of "Part:BBa K590052"
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<partinfo>BBa_K590052 short</partinfo> | <partinfo>BBa_K590052 short</partinfo> | ||
− | This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase (ADC) | + | This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase] (ADC) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase] (AAR), in a linear configuration with a glycine-serine linker for co-localization. |
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 20:38, 28 September 2011
Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) Fusion
This part encodes a direct gene fusion product of the two enzymes, Aldehyde Decarbonylase (ADC) and Acyl-ACP Reductase (AAR), in a linear configuration with a glycine-serine linker for co-localization.
Usage and Biology
We expected this will optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR close together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production relative to previous work with independent expression constructs. The functional assembly was put under a high-copy constitutive promoter and can be found under the BBa_K590049 part page.
For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]