Difference between revisions of "Part:BBa K590026"
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This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase], from ''Synechococcus elongatus'', in PSB1C3 high consititutive vector. This was an important negative control in testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick] - which consists of Aldehyde Decarbonylase and another part from ''Synechococcus elongatus'', [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 Acyl-ACP Reductase]. | This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase], from ''Synechococcus elongatus'', in PSB1C3 high consititutive vector. This was an important negative control in testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick] - which consists of Aldehyde Decarbonylase and another part from ''Synechococcus elongatus'', [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 Acyl-ACP Reductase]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 20:06, 28 September 2011
ADC-PSB1C3-High consititutive
This part consists of Aldehyde Decarbonylase, from Synechococcus elongatus, in PSB1C3 high consititutive vector. This was an important negative control in testing the PetroBrick - which consists of Aldehyde Decarbonylase and another part from Synechococcus elongatus, Acyl-ACP Reductase.
Usage and Biology
This part produces ADC, which is the second step in the conversion of cellular fatty acyl-ACPs to alkanes. ADC decarbonylates the aldehyde produced in the first step by Acyl-ACP Reductase. This part is best used in combination with some kind of reductase, to form alkanes from the aldehydes made by the reductase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1254
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]