Difference between revisions of "Part:BBa K542007"

Revision as of 00:41, 29 September 2011

Unregulated TetR with pTet Regulated Arg-tagged ECFP

Made by assembling BBa_P0440 with BBa_K331033.

The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter (BBa_R0010) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.

Intermediate part to assemble the "Co-localization Testing Construct".

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 8: / Line 8: @@
 Intermediate part to assemble the "Co-localization Testing Construct".
 <br><br>
-Datasheet for the test construct <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct.
+This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
 <br><br>
 [[image:uoflECdatasheet.png|150px]]