Difference between revisions of "Part:BBa K542004"

Revision as of 18:45, 28 September 2011

pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD

Made by assembling BBa_K542001 with BBa_K542003.

Note that the pLacI promoter (BBa_R0010) is regulating the Lumazine Synthase gene, not the pBAD promoter (BBa_I13453) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible.
The microcompartment portion to the "Co-localization Construct".

Datasheet for the test construct Part BBa_K542008 in E. coli strain DH5alpha demonstrating this is a functional intermediate in that construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 961
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 901
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 The microcompartment portion to the "Co-localization Construct".
 <br><br>
-Datasheet for the completed <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha.
+Datasheet for the test construct <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct.
 <br><br>
 [[image:uoflECdatasheet.png|150px]]