Difference between revisions of "Part:BBa K620001"
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===Usage and Biology===
 
===Usage and Biology===
 
[[Image:P450psb1c3.png|frame|center|Plasmid map of K620001 in pSB1C3 (submission plasmid).]]
 
[[Image:P450psb1c3.png|frame|center|Plasmid map of K620001 in pSB1C3 (submission plasmid).]]
 +
[[BPA_electrospray|frame|center|GCMS-electrospray of BPA showing characteristic peak at 227g/mole]]
 +
[[Image:BPAdegra.png|frame|center|GCMS of BPA after reaction with the WT-F87A P450 that shows a degraded peak at 205 g/mole]]
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 23:27, 28 September 2011

WT-F87A (p450)

A wild-type p450 with a mutation at the 87th amino acid. It is confirmed to degrade bisphenol A (BPA), but as it is a promiscuous protein, it can likely bind and degrade other organic molecules.


Usage and Biology

Plasmid map of K620001 in pSB1C3 (submission plasmid).

frame|center|GCMS-electrospray of BPA showing characteristic peak at 227g/mole

GCMS of BPA after reaction with the WT-F87A P450 that shows a degraded peak at 205 g/mole

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 75
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1219
    Illegal SapI.rc site found at 2242
    Illegal SapI.rc site found at 2842