Difference between revisions of "Part:BBa K566012"
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===Usage and Biology=== | ===Usage and Biology=== | ||
− | The lambda repressor is a dimeric protein also known as the cI protein. cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. | + | The lambda repressor is a dimeric protein also known as the cI protein. cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. Modified with a LVA tag for rapid degradation of the protein |
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Revision as of 20:07, 28 September 2011
cI repressor from Lambda phage optimized for E. coli cI (+LVA)
cI repressor from Lambda phage optimized for E. coli. Includes LVA tag.
Preferential codons to improve their expression in E. coli.
Usage and Biology
The lambda repressor is a dimeric protein also known as the cI protein. cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. Modified with a LVA tag for rapid degradation of the protein
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 463
- 1000COMPATIBLE WITH RFC[1000]