Difference between revisions of "Part:BBa K578003"

 
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<partinfo>BBa_K578003 short</partinfo>
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K578003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K578003 SequenceAndFeatures</partinfo>
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===Confirmation of Part Length===
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[[Image:ASU_RSR.png|400px]]
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===Use of this BioBrick===
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This part is intended for use with [https://parts.igem.org/Part:BBa_K578002 BBa_K578002] to build up a repeat/spacer array that targets GFP expression.
  
  

Revision as of 23:38, 2 October 2011

RSR Array Segment (Listeria innocua CLIP11262)

This brick, T7LRSR, is the starting point for a CRISPR (Clustered Regularly Interspaced Short Palindromic Repeat) array based around Listeria Innocua CLIP11262. The sequence of the part is composed of a T7 promoter, Lac Operator, Leader Sequence (from Listeria Innocua CLIP11262), Palindromic Repeat, Spacer (Targeted to sequence with correct protospacer and 'NGG; Protospacer-adjacent motif), followed by a palindromic repeat. After the aforementioned sequences there is a pseudospacer with the standard BioBrick suffix (with SpeI and PstI Sites). The suffix allows for parts (eg.Repeat.Spacer Repeat, RSR, formated BioBricks) to be added onto the distal end of the CRISPR array.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Confirmation of Part Length

ASU RSR.png

Use of this BioBrick

This part is intended for use with BBa_K578002 to build up a repeat/spacer array that targets GFP expression.