Difference between revisions of "Part:BBa K633003"

 
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Sacc Extracellular Sucrase
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'''Sacc Extracellular Sucrase'''
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This extracellular sucrase is an enzyme from Zymomonas mobilis. The expression product is different from SacB; showing sucrase but not levansucrase activity. In E. coli BL21, after induction of the T7 polymerase expression system with IPTG. Subcellular fractionation of the E. coli clone carrying plasmid pLSS2811 showed that more than 70% of the sucrase activity could be detected in the cytoplasmic fraction, suggesting that the enzyme was soluble and not secreted in E. coli. Its structure is monomeric, with an optimum temperature 20 to 40 ° C, pH of 2.5-7.5 and a size of 48 kDa. It also has a free N-terminus.
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'''Kannan R, Mukundan G, Aït-Abdelkader N, Augier-Magro V, Baratti J, Gunasekaran P. Molecular cloning and characterization of the extracellular sucrase gene (sacC) of Zymomonas mobilis. Arch Microbiol. 1995 Mar;163(3):195-204.'''
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Revision as of 17:28, 17 October 2011

Extracellular Succrase Sacc

Sacc Extracellular Sucrase



This extracellular sucrase is an enzyme from Zymomonas mobilis. The expression product is different from SacB; showing sucrase but not levansucrase activity. In E. coli BL21, after induction of the T7 polymerase expression system with IPTG. Subcellular fractionation of the E. coli clone carrying plasmid pLSS2811 showed that more than 70% of the sucrase activity could be detected in the cytoplasmic fraction, suggesting that the enzyme was soluble and not secreted in E. coli. Its structure is monomeric, with an optimum temperature 20 to 40 ° C, pH of 2.5-7.5 and a size of 48 kDa. It also has a free N-terminus.


Kannan R, Mukundan G, Aït-Abdelkader N, Augier-Magro V, Baratti J, Gunasekaran P. Molecular cloning and characterization of the extracellular sucrase gene (sacC) of Zymomonas mobilis. Arch Microbiol. 1995 Mar;163(3):195-204.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 907
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 607