Difference between revisions of "Part:BBa K569011:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | '''FGFR''' | |
| − | + | - 1. Added restriction sites XbaI and SpeI via PCR mechanism | |
| − | + | - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene | |
===Source=== | ===Source=== | ||
Revision as of 01:09, 29 September 2011
FGFR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 587
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 165
Illegal NgoMIV site found at 756 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
FGFR
- 1. Added restriction sites XbaI and SpeI via PCR mechanism - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
Source
?