Difference between revisions of "Part:BBa K569010:Design"

 
(→‎Design Notes)
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K569010 short</partinfo>
 
<partinfo>BBa_K569010 short</partinfo>
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===Design Notes===
 
===Design Notes===
−
Na
+
  CTX
−
 
+
            foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
−
 
+
            reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'
 +
  CheZ
 +
          5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3'
 +
          5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'
 +
          to add the BioBrick restriction enzymes sites.
  
 
===Source===
 
===Source===

Revision as of 01:02, 29 September 2011

ctx+CheZ mutant


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 818


Design Notes

 CTX
           foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
           reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'
 CheZ 
          5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' 
          5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3' 
          to add the BioBrick restriction enzymes sites.

Source

Na

References