Difference between revisions of "Part:BBa K566000"

Revision as of 19:54, 27 September 2011

OL region from Lambda

OL region from Lambda phage. Allows biphasic switch (K566002) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter (K566001).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K566000 short</partinfo>
-OL region from Lambda phage. Allows biphasic switch [https://parts.igem.org/Part:BBa_K566002 (K566002)] effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter [https://parts.igem.org/Part:BBa_K566001 (K566001)].
+OL region from Lambda phage. Allows biphasic switch ([https://parts.igem.org/Part:BBa_K566002 K566002]) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter ([https://parts.igem.org/Part:BBa_K566001 K566001]).
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