Difference between revisions of "Part:BBa K598014"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K598014 short</partinfo>
 
<partinfo>BBa_K598014 short</partinfo>
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This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
 
This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.
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It is the positive control of the theophylline inducing experiment. For further information, please see ，[https://parts.igem.org/Part:BBa_K598013#Reference BBa_K598013].
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 14:08, 5 October 2011

pBAD+HH+CdIntron+E0040+b0015


This a GFP reporter regulated by a self-cleaving hammerhead ribozyme. The self-cleaving of the hammerhead ribozyme would lead to the formation of the intron P1 stem, thus triggering the self-splicing of the intron.

It is the positive control of the theophylline inducing experiment. For further information, please see ，BBa_K598013.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1408