Difference between revisions of "Part:BBa K634007"
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<partinfo>BBa_K634007 short</partinfo>
 
<partinfo>BBa_K634007 short</partinfo>
  
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When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways:
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For additional detailed information, characterization, and usage, see [[Part:BBa_K634007:Experience|the experience page]].
  
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Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence)
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'''Purpose'''
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This device can serve three roles (fluorescent reporter, selectable marker, counter-selectable marker) in an ''E. coli''-based system. The consolidation of these functions to a single device should facilitate directed evolution studies. A sample usage of K634007 is presented below to clarify. This part is intended to be cloned downstream of one- or two-component sensor systems, to be expressed as (or in tandem with) the reporter. The most benefit can be derived from this part in studies involving large mutant libraries of sensor components, such as the product of an error-prone PCR amplification of a sensor kinase.
  
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Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin.
 
  
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Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose
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'''Use case'''
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 22:17, 27 September 2011

Double selection cassette (tagRFP-sacB-kanR)

For additional detailed information, characterization, and usage, see the experience page.

Purpose This device can serve three roles (fluorescent reporter, selectable marker, counter-selectable marker) in an E. coli-based system. The consolidation of these functions to a single device should facilitate directed evolution studies. A sample usage of K634007 is presented below to clarify. This part is intended to be cloned downstream of one- or two-component sensor systems, to be expressed as (or in tandem with) the reporter. The most benefit can be derived from this part in studies involving large mutant libraries of sensor components, such as the product of an error-prone PCR amplification of a sensor kinase.


Use case


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 648
    Illegal SapI.rc site found at 30