Difference between revisions of "Part:BBa K530001"

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(→‎Sequencing)
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[[Image:CrtE Sequencing.png]]
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This is the sequencing for colony 7. Colony 7 had a perfect match with the genomic DNA promoter.
 
This is the sequencing for colony 7. Colony 7 had a perfect match with the genomic DNA promoter.

Revision as of 17:02, 28 October 2011

CRTE

Enzyme in the pathway required for B-Carotene Synthesis. This enzyme is named Geranylgeranyl Diphosphate Synthase. This sequence was taken from a WT strain of xanthophyllomyces dendrorhous. It catalyzes both the conversion of Geranylgeranyl diphosphate to Phytoene and Lycopene to B-Carotene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 335
    Illegal XhoI site found at 34
    Illegal XhoI site found at 166
    Illegal XhoI site found at 985
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 202
  • 1000
    COMPATIBLE WITH RFC[1000]


Plasmid Map

CrtE Plasmid Map.png


Sequencing

CrtE Sequencing 2.png


This is the sequencing for colony 7. Colony 7 had a perfect match with the genomic DNA promoter.