Difference between revisions of "Part:BBa K566021"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K566021 short</partinfo> | <partinfo>BBa_K566021 short</partinfo> | ||
− | pcyA CDS | + | pcyA (Phycocyanobilin:ferredoxin oxidoreductase) CDS |
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+ | Gene in charge of the cromophore synthesis along with ho1 in <i>Synechocystis</i> which convert heme into PCB via a two-step 145 oxidation/reduction process. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Is the best characterized member of the ferredoxin-dependent bilin reductase family. Unlike other ferredoxin-dependent bilin reductases that catalyze a two-electron reduction, PcyA sequentially reduces D-ring (exo) and A-ring (endo) vinyl groups of biliverdin IXalpha (BV) to yield phycocyanobilin, a key pigment precursor of the light-harvesting antennae complexes of red algae, cyanobacteria, and cryptophytes. | ||
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+ | [[Image:Captura_de_pantalla_2011-09-28_a_las_19.11.40.png|thumb|center|500px|''' | ||
+ | FIGURE 1.Reactions catalyzed by PcyA. A, PcyA reduces BV to 3Z/3E-PCB via an intermediate of 18EtBV in two steps. Two electrons per each step are supplied from ferredoxin. B, when BV13, an isomer of BV, is used as the substrate, PcyA reduces it to 3Z/3E-iso-PΦB using two electrons. Fukuyama K. <i>et al</i> (2010) Structural Insights into Vinyl Reduction Regiospecificity of Phycocyanobilin:Ferredoxin Oxidoreductase (PcyA). <i>J Biol Chem.</i> 2010 January 8; 285(2): 1000–1007.]] | ||
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Revision as of 00:20, 29 September 2011
pcyA CDS
pcyA (Phycocyanobilin:ferredoxin oxidoreductase) CDS
Gene in charge of the cromophore synthesis along with ho1 in Synechocystis which convert heme into PCB via a two-step 145 oxidation/reduction process.
Usage and Biology
Is the best characterized member of the ferredoxin-dependent bilin reductase family. Unlike other ferredoxin-dependent bilin reductases that catalyze a two-electron reduction, PcyA sequentially reduces D-ring (exo) and A-ring (endo) vinyl groups of biliverdin IXalpha (BV) to yield phycocyanobilin, a key pigment precursor of the light-harvesting antennae complexes of red algae, cyanobacteria, and cryptophytes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 322
- 1000COMPATIBLE WITH RFC[1000]