Difference between revisions of "Part:BBa K640002"
Emily Hicks (Talk | contribs) |
|||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K640002 short</partinfo> | <partinfo>BBa_K640002 short</partinfo> | ||
| − | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
| Line 17: | Line 16: | ||
<partinfo>BBa_K640002 parameters</partinfo> | <partinfo>BBa_K640002 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | |||
| + | <h3>Functionality Test</h3> | ||
| + | |||
| + | In our first assay, we wanted to see that our BBa_K640004 (oriT-ori1600) construct was working. We incubated the co-cutures for 12 hours at 37°c prior to plating. The plates were then left to grow overnight. | ||
| + | |||
| + | <h3>Results</h3> | ||
| + | |||
| + | [[image:Ucalgary_2011_MacConkiePlates.png]] | ||
| + | <br><br> | ||
| + | The large plates on the right show controls of <i>Pseudomonas</i> and <i>E. coli</i> on MacConkey plates indicating the normal expected colors for these organisms. The small plates on the bottom represent our construct, conjugated with two different strains of pseudomonas and plated on MacConkey agar with Kanamycin (50ug/mL). The yellow color indicates that the colonies are <i>Pseudomonas</i>. The small plates on the top show our two strains of <i>Pseudomonas</i>, as well as <i>E. coli</i> without kanamycin resistance plated on MacConkey agar plates with kanamycin (50 ug/mL). All of these plates are pink in color, and show no colonies. This was expected, as it shows that without a resistance marker, these organisms are not able to grow on the MacConkey kanamycin plates. This demonstrates that our part is working the way we expect it to! | ||
Revision as of 04:29, 29 September 2011
oriT - Interspecies origin of transfer
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functionality Test
In our first assay, we wanted to see that our BBa_K640004 (oriT-ori1600) construct was working. We incubated the co-cutures for 12 hours at 37°c prior to plating. The plates were then left to grow overnight.
Results
The large plates on the right show controls of Pseudomonas and E. coli on MacConkey plates indicating the normal expected colors for these organisms. The small plates on the bottom represent our construct, conjugated with two different strains of pseudomonas and plated on MacConkey agar with Kanamycin (50ug/mL). The yellow color indicates that the colonies are Pseudomonas. The small plates on the top show our two strains of Pseudomonas, as well as E. coli without kanamycin resistance plated on MacConkey agar plates with kanamycin (50 ug/mL). All of these plates are pink in color, and show no colonies. This was expected, as it shows that without a resistance marker, these organisms are not able to grow on the MacConkey kanamycin plates. This demonstrates that our part is working the way we expect it to!