Difference between revisions of "Part:BBa K615003:Design"
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===Design Notes=== | ===Design Notes=== | ||
rpoZ knocked out using lambda red | rpoZ knocked out using lambda red | ||
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Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC | Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC | ||
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===Source=== | ===Source=== | ||
Revision as of 03:46, 27 September 2011
E. coli strain for TolC one-hybrid selection system
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 632
Illegal PstI site found at 952 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 632
Illegal PstI site found at 952 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 845
Illegal BglII site found at 965 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 632
Illegal PstI site found at 952 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 632
Illegal PstI site found at 952
Illegal NgoMIV site found at 181 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 121
Illegal SapI site found at 331
Design Notes
rpoZ knocked out using lambda red
Kanamycin cassette-Zif268 binding site-weak promoter placed upstream of endogenous TolC
Source
Based off EcNR2 selection strain (see Wang et al, 2009)