Difference between revisions of "Part:BBa K566008:Design"
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===References=== | ===References=== | ||
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+ | Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro | ||
+ | <b>1.</b> Andrew K. Vershon, Sha-Mei Liao, William R. McClure and Robert T. Sauer (1987) Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro. <i>J. Mol. Riol</i>. (1987) 195, 311-322 |
Revision as of 19:55, 28 September 2011
Mnt repressor optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 228
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 148
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Search for illegal sites was performed, they were found and removed.
Source
Original sequence was taken from BioBrick BBa_C0072, this was optimized with preferential codons for better expression into E. coli.
References
Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro 1. Andrew K. Vershon, Sha-Mei Liao, William R. McClure and Robert T. Sauer (1987) Bacteriophage P22 Mnt Repressor DNA Binding and Effects on Transcription in Vitro. J. Mol. Riol. (1987) 195, 311-322