Difference between revisions of "Part:BBa K634007"
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When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways: | When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways: | ||
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Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence) | Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence) | ||
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Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin. | Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin. | ||
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Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose | Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose | ||
Revision as of 03:51, 27 September 2011
Double selection cassette (tagRFP-sacB-kanR)
When placed 3' of the promoter portion of a 1- or 2-component sensor, BBa_634007 can be used in the following ways:
Reporter: tagRFP (BBa_K634005) fluoresces red upon induction (see BBa_K634005 for issues with fluorescence)
Selection: Sensor systems can be selected for desired induced expression through supplementation of the culture with the sensor's analyte and kanamycin.
Counter selection: Sensors can also be selected for absence of expression when uninduced through supplementation of the culture with sucrose
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 648
Illegal SapI.rc site found at 30