Difference between revisions of "Part:BBa K566004:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ===Experience=== |
| + | Testing and characterization for composite K566004 and K566006 in E.coli JT2 strain under different light intensities and irradiation times of 30 minutes at 532 nm. | ||
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| + | Experimental | ||
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| + | G (+) = E.coli JT2 containing pJT122, pPLPCB(S), K566004 (CFP), K566006 (Green light genetic inversor) | ||
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| + | G (-) = E.coli JT2 containing pJT122, K566004(CFP), K566006 (Green light genetic inversor) | ||
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| + | A light irradiation experiment was carried out in JT2 cultures under two controlled environments, one exposed to light and the other in complete darkness. The samples G (+) and G (-) were incubated overnight at 37°C and then 200uL of the samples were inoculated by triplicate for each intensity. | ||
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| + | Both samples were measured in a Fluorometer BioTek Synergy, using special filters for CFP detection and measurement (Exitation filter 420nm ±50 and Emsion filter 460 ±40) before being irradiated to observe an initial fluorescence. After been introduced to the Light Machine, where the exposure time started with 30 minutes of Far-red light(710 nm) to reset the photoreceptors that could be switched during the night at the end of this period the fluorescence was measured. Then began to expose the samples to green light (532 nm) for 2 hours and every 30 minutes a portion of the sample is taken to measure the fluorescence gained or lost. | ||
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| + | [[Image:CFPgraficauno.png|thumb|center|500px|]] | ||
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| + | [[Image:CFPgraficados.png|thumb|center|500px|'''Figure 2. CFP tends to increase over time.]] | ||
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| + | Results | ||
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| + | At the end of two hours the following data was obtained: | ||
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| + | Plate Control (-) | ||
| + | **Always in the dark | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 04:05, 29 September 2011
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Experience
Testing and characterization for composite K566004 and K566006 in E.coli JT2 strain under different light intensities and irradiation times of 30 minutes at 532 nm.
Experimental
G (+) = E.coli JT2 containing pJT122, pPLPCB(S), K566004 (CFP), K566006 (Green light genetic inversor)
G (-) = E.coli JT2 containing pJT122, K566004(CFP), K566006 (Green light genetic inversor)
A light irradiation experiment was carried out in JT2 cultures under two controlled environments, one exposed to light and the other in complete darkness. The samples G (+) and G (-) were incubated overnight at 37°C and then 200uL of the samples were inoculated by triplicate for each intensity.
Both samples were measured in a Fluorometer BioTek Synergy, using special filters for CFP detection and measurement (Exitation filter 420nm ±50 and Emsion filter 460 ±40) before being irradiated to observe an initial fluorescence. After been introduced to the Light Machine, where the exposure time started with 30 minutes of Far-red light(710 nm) to reset the photoreceptors that could be switched during the night at the end of this period the fluorescence was measured. Then began to expose the samples to green light (532 nm) for 2 hours and every 30 minutes a portion of the sample is taken to measure the fluorescence gained or lost.
Results
At the end of two hours the following data was obtained:
Plate Control (-)
- Always in the dark
User Reviews
UNIQd7f71dfc5fc7bc36-partinfo-00000000-QINU UNIQd7f71dfc5fc7bc36-partinfo-00000001-QINU