Difference between revisions of "Part:BBa K526000:Design"

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tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggacttactagagtcacacaggacggccgggtgagtaaacgtcgttatcttaccggtaaagaagttcaggccatgatgcaggcggtttgttacggggcaacgggagccagagattattgtcttattctgttggcatatcggcatgggatgcgtattagtgaactgcttgatctgcattatcaggaccttgaccttaatgaaggtagaataaatattcgccgactgaagaacggattttctaccgttcacccgttacgttttgatgagcgtgaagccgtggaacgctggacccaggaacgtgctaactggaaaggcgctgaccggactgacgctatatttatttctcgccgcgggagtcggctttctcgccagcaggcctatcgcattattcgcgatgccggtattgaagctggaaccgtaacgcagactcatcctcatatgttaaggcatgcttgcggttatgaattggcggagcgtggtgcagatactcgtttaattcaggattatctcgggcatcgaaatattcgccatactgtgcgttataccgccagtaatgctgctcgttttgccggattatgggaaagaaataatctcataaacgaaaaattaaaaagagaagaggtttga
 
  
  

Revision as of 07:42, 26 September 2011

Pompc promoter coding fimE


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 750
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 750
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 750
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 750
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 750
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

There is no special design consideration. We can amplify the Pompc and fimE from E. coli genomic DNA and assembly them using Gibson's assembly.

Source

Both fimE and Pompc was amplified from E. coli genomic DNA.

Ham, T.S., Lee, S.K., Keasling, J.D. and Arkin, A.P. A tightly regulated inducible expression system utilizing the fim inversion recombination switch. Biotechnology and Bioengineering 94 (2006), pp. 1-4.

References