Difference between revisions of "Part:BBa K575029"

Line 17: Line 17:
 
<partinfo>BBa_K575029 parameters</partinfo>
 
<partinfo>BBa_K575029 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===Characterization TokyoTech 2019===
 +
Author:iGEM TokyoTech2019 (https://2019.igem.org/Team:TokyoTech)

Revision as of 13:26, 21 October 2019

LasR/PAI1 Inducible Promoter + RBS (B0034) + GFP, Constitutive Promoter + RBS (B0034) + LasR

Continuous expression of LasR (with RBS B0034), coupled with a LasR/PAI1 (3-oxo-C12-HSL) inducible promoter, RBS (Part B0034), and a GFP reporter.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 918
    Illegal NheI site found at 941
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1294
    Illegal AgeI site found at 1491
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 827


Characterization TokyoTech 2019

Author:iGEM TokyoTech2019 (https://2019.igem.org/Team:TokyoTech)