Difference between revisions of "Part:BBa K575026"
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<partinfo>BBa_K575026 parameters</partinfo>
 
<partinfo>BBa_K575026 parameters</partinfo>
 
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[http://2018.igem.org/Team:RMHS_Maryland Team RMHS_Maryland 2018] contributed to the characterization of this part by demonstrating that the part produces RFP expression when induced by AI-1. 
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(<small>--[[User:ishayardi|ishayardi]] 20:24, 16 October 2018 (UTC)</small>)
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==Team RMHS_Maryland 2018: Characterization of AI-1 Induced Fluorescence in E. Coli  <i>Escherichia coli</i> for use in co-culture==
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Revision as of 01:13, 17 October 2018

LasR/PAI1 Inducible Promoter + RBS (B0030) + RFP, Constitutive Promoter + RBS (B0030) + LasR

This construct contains a LasR/PaI1 (3-oxo-C12-HSL) inducible promoter with an RBS and an RFP reporter. There is also constitutive transcription of the LasR receptor protein. Thus, the promoter will activate and fluorescence will be observed in the presence of PAI1.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 58
    Illegal NheI site found at 907
    Illegal NheI site found at 930
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1286
    Illegal AgeI site found at 741
    Illegal AgeI site found at 853
    Illegal AgeI site found at 1483
  • 1000
    COMPATIBLE WITH RFC[1000]


[http://2018.igem.org/Team:RMHS_Maryland Team RMHS_Maryland 2018] contributed to the characterization of this part by demonstrating that the part produces RFP expression when induced by AI-1.
(--ishayardi 20:24, 16 October 2018 (UTC))

Team RMHS_Maryland 2018: Characterization of AI-1 Induced Fluorescence in E. Coli Escherichia coli for use in co-culture