Difference between revisions of "Part:BBa K537001:Experience"
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<partinfo>BBa_K249026</partinfo>
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<I>Experience by Wits-CSIR_SA iGEM2011</I>
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<p> Team Lethbridge 2009, used the Gallivan theophylline riboswitch (clone 8.1) to generate a riboswitch-CheZ construct. However, this team created a 11 nt insert after the ATG start codon. This result created a TAG stop codon (in the 3rd amino acid position) as well as a frameshift mutation. Therefore it is unlikely that a functional CheZ could have been produced from this system. (see team Lethbridge 2009 below, riboswitch highlighted in yellow, inserted biobrick “scar” region highlighted in violet, ATG start underlined)</p>
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<html><img src="https://static.igem.org/mediawiki/2011/6/6c/Wits_Overview_Sequence_Yellow_2.jpg" width="500"></html>
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<p>[[BBa_K249026]] - Team Lethbridge iGEM2009</p>
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<p>For more detailed analysis of how this part can be improved, visit our results page[http://2011.igem.org/Team:WITS-CSIR_SA/Project/Concept] and the following parts:[[BBa_K537001]] [[BBa_K537003]] </p>
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Revision as of 17:30, 25 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.!

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UNIQec70d7ec697001d4-partinfo-00000000-QINU UNIQec70d7ec697001d4-partinfo-00000001-QINU