Difference between revisions of "Part:BBa K560000"
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<partinfo>BBa_K560000 short</partinfo>
 
<partinfo>BBa_K560000 short</partinfo>
  
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LovTAP Photosensor is a chimeric protein. It is built by fusing LOV domain, which senses blue light, to the trpR-DNA binding domain of the transcription factor trp repressor.
 
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Under the presence of blue light, maximum at 450nm, the absorption of a photon leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.
 
  
  
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===Usage and Biology===
 
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K560000 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K560000 SequenceAndFeatures</partinfo>
  
  
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===Functional Parameters===
 
===Functional Parameters===
 
<partinfo>BBa_K560000 parameters</partinfo>
 
<partinfo>BBa_K560000 parameters</partinfo>
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Revision as of 14:56, 30 September 2011

Blue Light Sensor+PtrpL+mRFP1




Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 918
    Illegal SpeI site found at 926
    Illegal AgeI site found at 1525
    Illegal AgeI site found at 1637
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 595


Functional Parameters

n/a Blue Light Sensor+PtrpL+mRFP1