Difference between revisions of "Part:BBa K518000"
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We performed a calibration for Photynus pyralis (Firefly) luciferase using standard reagents of enzyme and D-luciferin. Reagents were granted from Berthold Japan (Tokyo, Japan). | We performed a calibration for Photynus pyralis (Firefly) luciferase using standard reagents of enzyme and D-luciferin. Reagents were granted from Berthold Japan (Tokyo, Japan). | ||
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===Usage=== | ===Usage=== | ||
Revision as of 12:35, 25 September 2011
RBS + firefly luciferase + d.terminator
Luciferase from firyfly (Photinus pyralis) expression cassette. Firefly luciferase emits light by the oxidation of D-luciferin, its substrate. Luciferase can be used as a measuring tool when combined with other cis-elements (regions regulating the expression of genes on the same DNA molecule; promoters and other protein-binding domains), because of its highly quantitative performance.
Calibration
We performed a calibration for Photynus pyralis (Firefly) luciferase using standard reagents of enzyme and D-luciferin. Reagents were granted from Berthold Japan (Tokyo, Japan).
Usage
For detailed data obtained, see [http://2011.igem.org/Team:UT-Tokyo our page] and BBa_K518002.
Reference
Bronstein I., et.al., Chemiluminescent and Bioluminescent reporter gene assays. Anal. Biochem, 219, 169 (1994). DeLuca M., et al., Firefly Luciferase: Mechanism of action, cloning and expression of the active enzyme. J. Biolum. Chemilum. 3, 1 (1989). Gould, S.J. and S. Subramani, Firefly luciferase as a tool in molecular and cell biology. Anal. Biochem. 175:5-13 (1988)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 827