Difference between revisions of "Part:BBa K611011"

Latest revision as of 01:23, 25 September 2011

cI lambda Repressor Characterization Construct

This part is used to characterize mutants of BBa_C0051 (cI). A mutant of BBa_C0051 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI and PstI. One can then measure several of the mutant's properties through its regulation of GFP expression. Concentration of the added cI mutant can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1065
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1005
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 719

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 <partinfo>BBa_K611011 short</partinfo>
-This part is used to characterize mutants of BBa_C0051 (cI). A mutant of BBa_C0051 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI PstI. One can then measure several of the mutant's properties through its regulation of GFP expression. Concentration of the added cI mutant can be varied through different arabinose concentrations as long as AraC is present.
+This part is used to characterize mutants of BBa_C0051 (cI). A mutant of BBa_C0051 can be ligated onto the 3' end of the part by cutting this plasmid at SpeI and PstI and by cutting the mutant repressor at XbaI and PstI. One can then measure several of the mutant's properties through its regulation of GFP expression. Concentration of the added cI mutant can be varied through different arabinose concentrations as long as AraC is present.
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